Normal serum folate.
نویسندگان
چکیده
منابع مشابه
Purification of folate binding factor in normal umbilical cord serum.
Human umbilical cord serum was found to contain both free folate and folate complexed to a high-molecular weight factor. The complexed folate was bound to a very high affinity binder and was present in concentrations equivalent to as much as 60 ng of 5-methyltetrahydrofolic acid per ml of serum. Acidification of the serum caused disassociation of the folate-binder complex. Released folates were...
متن کاملRadioassay of serum folate.
We describe a radioassay procedure for serum folate, in which commercially available crystalline 3lactoglobulirt is used as the folate-binding agent. The folate binders present in serum are eliminated by heating serum in a boiling water bath for 15 mm to denature the proteins. Radioassayable serum folate is shown to be stable under these conditions, and the results for folate compare well with ...
متن کاملRadioassay of serum folate, with use of a serum blank and nondialyzed milk as folate binder.
Serum folate determination by this method requires incubation of 0.1 ml of serum (or standards) with nondialyzed milk and then with tritium-labeled folate. A serum blank corrects for nonspecific binders present in the serum. Unbound folate is removed with hemoglobin-coated charcoal. The standard deviation of the results is ±G.2 pig/liter, and more than 90% of folate added to the serum is accoun...
متن کاملObservations on Serum B1 and Folate Activity in Normal and Leukemic Rats
By THOMAS J. LYNCH AND WILLIAM C. MOLONEY D URING STUDIES on the induction of myelogenous leukemia in the rat, serum B12 afl(I folate activities were investigated. In man it is well known tiiat serum B1 activity is greatly elevated in chronic myelogenous leukemia ( CML ) . Less is known concerning serum folate activity hut from iiiitial reports and in our own experience, serum folate levels are...
متن کاملObservations on serum B-12 and folate activity in normal and leukemic rats.
By THOMAS J. LYNCH AND WILLIAM C. MOLONEY D URING STUDIES on the induction of myelogenous leukemia in the rat, serum B12 afl(I folate activities were investigated. In man it is well known tiiat serum B1 activity is greatly elevated in chronic myelogenous leukemia ( CML ) . Less is known concerning serum folate activity hut from iiiitial reports and in our own experience, serum folate levels are...
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ژورنال
عنوان ژورنال: BMJ
سال: 1968
ISSN: 0959-8138,1468-5833
DOI: 10.1136/bmj.2.5602.430-a